ruby - Performing a BLAST search with BioRuby -

i attempting perform blast search using bioruby on windows xp machine ruby 1.9.3 , bioruby 1.4.3_0001. have installed necessary dependencies, e.g., cairo, output follows:

c:/ruby193/lib/ruby/site_ruby/1.9.1/rubygems/core_ext/kernel_require.rb:45:in `r equire': cannot load such file -- cairo.so (loaderror)         c:/ruby193/lib/ruby/site_ruby/1.9.1/rubygems/core_ext/kernel_requir e.rb:45:in `require'         c:/ruby193/lib/ruby/gems/1.9.1/gems/cairo-1.12.6-x86-mingw32/lib/ca iro.rb:46:in `rescue in <top (required)>'         c:/ruby193/lib/ruby/gems/1.9.1/gems/cairo-1.12.6-x86-mingw32/lib/ca iro.rb:42:in `<top (required)>'         c:/ruby193/lib/ruby/site_ruby/1.9.1/rubygems/core_ext/kernel_requir e.rb:110:in `require'         c:/ruby193/lib/ruby/site_ruby/1.9.1/rubygems/core_ext/kernel_requir e.rb:110:in `rescue in require'         c:/ruby193/lib/ruby/site_ruby/1.9.1/rubygems/core_ext/kernel_requir e.rb:35:in `require'         c:/ruby193/lib/ruby/gems/1.9.1/gems/bio-graphics-1.4/lib/bio-graphi cs.rb:11:in `<top (required)>'         c:/ruby193/lib/ruby/site_ruby/1.9.1/rubygems/core_ext/kernel_requir e.rb:110:in `require'         c:/ruby193/lib/ruby/site_ruby/1.9.1/rubygems/core_ext/kernel_requir e.rb:110:in `rescue in require'         c:/ruby193/lib/ruby/site_ruby/1.9.1/rubygems/core_ext/kernel_requir e.rb:35:in `require'         bio283.rb:2:in `<main>' 

the sample code using follows:

require 'bio' require 'bio-graphics'  remote_blast_factory = bio::blast.remote('blastp', 'swissprot',                                          '-e 0.0001', 'genomenet')  seq = bio::sequence::aa.new('mfrtkrsalvrrlwrsrapggedeeegagggggggelrge')  # run actual blast querying factory report = remote_blast_factory.query(seq)  # then, parse report, see bio::blast::report report.each |hit|     puts hit.evalue           # e-value     puts hit.sw               # smith-waterman score (*)     puts hit.identity         # % identity     puts hit.overlap          # length of overlapping region     puts hit.query_id         # identifier of query sequence     puts hit.query_def        # definition(comment line) of query sequence     puts hit.query_len        # length of query sequence     puts hit.query_seq        # sequence of homologous region     puts hit.target_id        # identifier of hit sequence     puts hit.target_def       # definition(comment line) of hit sequence     puts hit.target_len       # length of hit sequence     puts hit.target_seq       # hit of homologous region of hit sequence     puts hit.query_start      # start position of homologous                               # region in query sequence     puts hit.query_end        # end position of homologous region                               # in query sequence     puts hit.target_start     # start position of homologous region                               # in hit(target) sequence     puts hit.target_end       # end position of homologous region                               # in hit(target) sequence     puts hit.lap_at           # array of above 4 numbers end 

could explain why problem occurring? noticed file name 'cairo.so' in output. relate linux/unix op. sys?

thanks,

caitlin

one of libraries installed depends on cairo. have install it. .so extension ruby library written in c, used in unix. in windows, need corresponding .dll file installed.